MARPLAST - Marine bacteria for bioplastic production

   Project MARPLAST
Marine bacteria for bioplastic production
Under ERA-NET Marine Biotechnology Transnational Cooperation
Contract no. 13/2017 University of Bucharest
Department of Genetics

Budget 195.000EURO
Duration: 36 months (march 2017-february 2020)
Finance Agency: RO-UEFISCDI
Romanian Team
Tanase Ana-Maria
Project responsible from UB
Stoica Ileana
Senior researcher, Prof. dr.
Vassu Tatiana
Senior researcher, Prof. dr.
Ionescu Robertina
Postdoc
Sarbu Ionela
Postdoc
Chiciudean Iulia  
PhD-student
Mereuta Ioana
PhD-student
Olteanu Amalia
Technician
Ionita Filuta
Financial responsible

PARTNERS IN THE PROJECT
Name of research organization
Short name
Organization type
Partner responsible
UiT – the Arctic University of Norway
UiT
UNI
Prof. Arne Smalas -project leader
Umea University
Uma
UNI
Prof. Knut Irgum
University of Bucharest
UB
UNI
Assoc. Prof. Ana-Maria Tanase
ABSTRACT
       The steady increase in microplastic concentration could result in dramatic effects on the vulnerable wildlife of the oceans and marine food supplies. It is therefore of immediate importance to develop novel types of polymeric materials that can be sustainably produced to address these environmental concerns. MARPLAST focuses on polyhydroxyalkanoates (PHAs), a class of biodegradable bioplastics which are considered to be feasible replacements for current petroleum-based plastics. PHAs are polymers occurring in nature, produced among others by bacteria, and with properties similar to oil-derived polypropylene and polyesters, rendering them useful as an attractive biodegradable replacement. However, the naturally occurring PHA production pathways are not sufficiently understood, and currently known technologies for production are too costly to allow for a full-scale replacement. MARPLAST aims to develop and provide tools (bacteria, enzymes, and pathways) to enable efficient production of sustainable and biodegradable bioplastics from low cost unexploited biomass. Focus will be on PHA-producing cold-adapted marine bacteria, which have a range of properties that make them especially suitable for industrial applications. MARPLAST will utilize expertise from the Univ of Tromsø (Norway), Univ of Bucharest (Romania) and Umeå University (Sweden) to make important progress and contributions to the transition to a bio-based European economy.
                On this focus of MARPLAST, Romanian Team will isolate new marine bacterial strains from Black Sea, especially those with a high PHA production but will test also all marine bacterial strains previously isolated and deposited to Microbial Collection of Department of Genetics. Bacterial strains will be phenotypically characterized using the Biolog Microbial Identification System and other usual microbiological tests. On molecular level, identification will be based on 16S rDNA sequences. Marine bacteria will be analyzed regarding PHA production by Red Nile fluorescence intensity measurements, in order to establish the best cultivation conditions for the highest production of PHA. Those conditions will be tested also to a larger scale in bioreactor.

PROLAB Etapa V / 2016

Rezultate etapa V/2016
Principalul obiectiv al acestei etape a constat în evaluarea funcţionalităţii consorţiului bazat pe tulpini de bacterii lactice (BL). Pentru atingerea acestui obiectiv au fost parcurse o serie de etape care au vizat:  analiza influenţei consorţiului asupra microbiotei umane simulate în sistemele GIS2; analiza din punct de vedere chimic a probelor prelevate din sistemele GIS2; analiza calitativă şi cantitativă a influenţei consorţiului  de BL asupra capacităţii de aderenţă a microorganismelor patogene la tumori prelevate de la nivelul tractului gastrointestinal; identificarea taxonomică a BL din consorţiu prin tehnici de biologie moleculară; evaluarea parametrilor biologici şi stabilităţii caracteristicilor probiotice în diferite condiţii de conservare şi stocare; îmbunătăţirea modelului experimental pentru obţinerea de produse probiotice complexe şi pentru anticiparea activităţii probiotice in vivo.
Obiectivele propuse în cadrul acestei etape au fost atinse şi au permis finalizarea proiectului cu selecţia unui consorţiu de BL cu efect probiotic. Consorţiul de BL, alcătuit din tulpinile: Lactobacillus (L.) casei, L. fermentum şi L. plantarum, a fost retestat privind capacitatea probiotică în vederea stabilirii condiţiilor optime de conservare.
Rezultate au fost diseminate prin publicare în 4 articole ISI (2 articole publicate şi 2 acceptate), un articol BDI, 2 participări la manifestãri ştiinţifice internaţionale si 2 brevete nationale.

Results phase V/2016
The main objective of this phase was to assess the functionality of probiotic lactic acid bacteria consortium.
To attain this major aim of the project, the research activities performed were focused on: influence of consortium on human microbiota simulated in GIS2 system, chemical analysis of samples taken from GIS2 system, qualitative and quantitative analysis of consortium’s influence on adherence ability of pathogenic microorganisms to human gastrointestinal tract tumors, taxonomic identification of consortium’s lactic acid bacteria strains by molecular techniques, evaluation of biological parameters and stability of probiotic characteristics in various conditions of preservation and storage, improving the experimental model in order to obtain complex probiotic products and to prepare in vivo tests for probiotic effect.
In conclusion, all the objectives of this stage were fully accomplished and one consortium was selected out of the 3 consortia obtained in the previous stages. The probiotic lactic acid bacteria consortium consisting of Lactobacillus (L.) casei, L. fermentum şi L. plantarum was retested for probiotic effect and to establish the optimal conditions of storage and preservation.
The results were disseminated through publication in 4 ISI papers (2 of them were published and 2 were accepted), 1 BDI paper, 2 participation in international conferences and 2 national patents.

Publication list
A.    Scientific articles published in ISI journals
1.      Mureşan A., Sârbu I., Pelinescu D., Ionescu R., Csutak O., Stoica I., Rusu E., Vassu T., 2016, In vitro selection of some lactic acid bacteria strains with probiotic potential. Romanian Biotechnological Letters, 23 (4). http://www.rombio.eu/published.html.
2.      Mureşan A., Sârbu I., Pelinescu D., Ionescu R., Csutak O., Stoica I., Rusu E., Vassu T., 2016, Virulence profiles of pathogenic bacterial strains isolated from different sources. Biointerface Research in Applied Chemistry; Vol. 6. http://biointerfaceresearch.com/?page_id=1657
3.      Zamfir M., Stefan I.R., Stancu M.M, Grosu-Tudor S.S., 2016. Production, mode of action and sequencing of the corresponding gene of a bacteriocin produced by Lactococcus lactis 19.3. Int J Food Sci Technol, 51: 2164–2170.
http://onlinelibrary.wiley.com/doi/10.1111/ijfs.13196/abstract
4.      Rusu E., Sârbu I., Cristescu C., Coculescu B.-I., Petruţ Ş., Mureşan A., Vassu T., Pelinescu D., 2016. Highlighting the antimicrobial activity of organic compounds isolated from some strains of lactic acid bacteria, Revista Romana de Chimie, nr. 12.

B.     Journals included in flow international BDI
1.      Vamanu Emanuel, The effect of a consortium probiotic administration on a microbiota microbiological stability, Lucrări ştiinţifice Seria Agronomie,  vol. 59/2016, pg. 1-4

C.    Scientific papers presented at international congresses / published in abstract
1.      Zamfir M., Stancu M.M., Ștefan I.R., Grosu-Tudor S.S. Characterization of a nisin-like bacteriocin produced by Lactococcus lactis 19.3, isolated from raw milk. Food Microbiology, Birmingham, UK, 7-10 august 2016, poster. – si de la proiectul PN-II-RU-TE-2014-4-0137
2.      Petruț S., Pelinescu D., Dițu L.M., Ionescu R., Vassu T., Stoica I., Chiciudean I., Tănase A.-M., Sârbu I., 2016. Comparative studies regarding probiotic effect between individual/consortia of lactic acid bacteria strains. “Power of Microbes in Industry and Environment 2016” Symposium, 28 Septembrie- 1 Octombrie, Krk, Croația.

D.    Patents
1.      Vamanu E., 2016, In vitro method for testing the viability of lactic acid bacteria probiotic strains during gastrointestinal tract transit, and unicameral system for its implementation.

2.      Vamanu E., 2016 A method of in vitro testing of the probiotic strains viability during colon transit and system for carrying out the method.

PROLAB 77 / 2015

Rezultate etapa IV/2015

În ultimii ani studiile realizate privind obţinerea de produse de tip probiotic şi elucidarea mecanismelor implicate în îmbunătăţirea stării de sănătate a consumatorului, au evidenţiat nu numai complexitatea interacţiunilor dintre microorganismele probiotice şi organismul gazdă (inclusiv microbiota de la nivelul tractului gastrointestinal), dar şi faptul că efectele sunt tulpină specifice. Pentru obţinerea unui efect probiotic cumulat şi potenţat în cadrul prezentului proiect obiectivul major este reprezentat de realizarea de consorţii de bacterii lactice cu efect probiotic.
Obiectivele operaţionale ale etapei IV-2015 au urmărit: caracterizarea compuşilor cu acţiune antimicrobiană produşi de bacterii lactice şi influenţa condiţiilor de creştere; influenţa consorţiilor asupra microbiotei umane simulate în sistemul GIS2; analiza din punct de vedere chimic a probelor prelevate din sistemele GIS2; analiza cantitativă a influenţei consorţiilor de bacterii lactice asupra profilului compuşilor secretaţi de celulele epiteliale şi evaluarea citotoxicitatii consorțiilor şi identificarea taxonomică a tulpinilor de bacterii lactice prin tehnici de biologie moleculară. 
Toate obiectivele propuse în cadrul prezentei etape au fost îndeplinite şi din cele 10 consorţii realizate în cadrul etapei anterioare, pe baza studiilor desfăşurate au fost selectate şi analizate 3 care au prezentat activitate antimicrobiană şi/sau activitate imunomodulatrie potenţată.
O parte din rezultate au fost diseminate prin publicare în 4 articole ISI (1 articol publicat şi 3 acceptate), 2 articole BDI, un brevet şi 4 participãri la manifestãri ştiinţifice internationale.
Este de menţionat faptul că sistemul GIS2 a fost premiat cu medalia de aur la salonul EuroInvent 2015.

Publicatii stiintifice:
Articole in reviste ISI
  1. Grosu-Tudor S.S., Zamfir M., Van der Meulen R., De Vuyst L., 2013, Isolation of novel homopolysaccharide-producing lactic acid bacteria from Romanian raw milk and fermented dairy products, European Food Research and Technology 237:609-615.
  2. Vamanu E., Pelinescu D.R., Avram I., Nita S., 2013, An in-vitro evaluation of antioxidant and colonic microbial profile levels following mushroom consumption, BioMed Research International http://dx.doi.org/10.1155/2013/289821
  3. Vamanu E., 2014, Testing in vitro viability of thermophilic probiotic strain in simulated gastrointestinal conditions,  Annals of Microbiology 64(3):1439-1442
  4. Grosu-Tudor S.-S., Stancu M.-M., Pelinescu D.,  Zamfir M., 2014, Characterization of some bacteriocins produced by lactic acid bacteria isolated from fermented foods, World Journal of Microbiology and Biotechnology 30: 2459–2469.
  5. Vamanu E., Sarbu I., Nedelcu I., Pelinescu D., 2015, Study of PROEXO product influence on infant microbiota in an in vitro colonic fermentation system, Annals of Microbiology 65(2): 1189-1193.
  6. Ditu L. M., Chifiriuc M. C., Pelinescu D., Sarbu I., Pircalabioru G., Mihaescu G., 2014, Class I and II Bacteriocins: Structure, Biosynthesis and Drug Delivery Systems for the Improvement of their Antimicrobial Activity, Current Proteomics  11: 121-127.
  7. Gatea F., Teodor E., Paun G., Matei A., Radu G. L., 2015, Capillary Electrophoresis Method Validation for Organic Acids Assessment in Probiotics, Food Analytical Method 8 (5): 1335-1340.
  8. Moroeanu V. I., Vamanu E., Paun G., Neagu E., Ungureanu O. R., Eremia S. A., Radu G. L, Ionescu R.,  Pelinescu D. R, 2015,  Probiotic strains influence on infant microbiota in the in vitro colonic fermentation model GIS1, Indian Journal of Microbiology, 55(4): 423-429
  9. Vamanu E, 2015,  Effect of gastric and small intestinal digestion on lactic acid bacteria activity in a GIS1 simulator, Saudi Journal of Biological Sciences– Elsevier, on-line paper
  10. Sarbu I., Vassu T., Stoica I., Marutescu L., Chifiriuc C.,  Pelinescu D., 2015,  Phenotypic and genotypic assessment of Lactobacillus plantarum influence on Candida albicans fluconazole resistance,  Annals of Microbiology, 1-7.
  11. Zamfir M., Stefan I., Grosu-Tudor S. S., 2015,  Influence of growth medium composition on the bacteriocin activity of some lactic acid bacteria, Romanian Biotechnological Letters, in press

Articole in reviste BDI
  1. Ditu L.M., Chifiriuc M. C.,  Marutescu L.,  Bleotu C.,  Pelinescu D.,  Bezirtzoglou E., Mihaescu G., Lazar V., 2014, Immunomodulatory effect of non-viable components of probiotic culture stimulated with heat-inactivated Escherichia coli and Bacillus cereus on holoxenic mice, Microbial Ecology In Health & Disease 25, p.1-6.
  2. Ditu L. M., Chifiriuc M.C., Pelinescu D., Sarbu I., Lazar V., Mihaescu G., 2014, Two-Component Regulatory Systems – implication in the quorum sensing mechanisms and bacteriocin production in lactic acid bacteria, Biointerface Research in Applied Chemistry  4(4), p. 813-815.
  3. Zamfir M., Cornea P.C., De Vuyst L., Grosu-Tudor S.-S., 2014, Biodiversity and biotechnological potential of lactic acid bacteria, AgroLife Scientific Journal 3(1), p. 169-176.
  4. Vamanu E., 2015, Biochemical properties and viability of two thermophilic lactic acid bacteria in a single chamber gastrointestinal tract simulator –GIS1: partial characterizations, University of Agricultural Sciences and Veterinary Medicine Iasi, Lucrări Ştiinţifice-Seria Zootehnie, 312 – 315.
  5. Sarbu I., Vassu T., Stoica I., Chifiriuc C. Bucur M., Rusu E., Pelinescu D., 2015, Analysis on the antimicrobial activity of some lactic acid bacteria strains, Journal of Infectious Diseases 18(2-3): 87-91.

Prezentari la conferinte 
  1. Grosu – Tudor S.S., Zamfir M., 2012,  Functional properties of lactic acid bacteria isolated from Romanian fermented vegetables, II International Conference on Antimicrobial Research, Lisabona, Portugalia, 21-23 noiembrie 2012, p. 563.
  2. Grosu-Tudor S.-S., Stancu M.-M., Pelinescu D.,  Zamfir M., 2014, Characterization of some bacteriocins produced by lactic acid bacteria isolated from fermented foods, 11th Symposium on Lactic Acid Bacteria, Egmond Aan Zee/Olanda, 31august-04 septembrie
  3. Sarbu I., Vassu T., Stoica  I., Marutescu L., Chifiriuc C., Nedelcu I., Pelinescu D., 2014, The influence of lactic acid bacteria strains on the antifungal resistance mechanisms of Candida albicans strains, 11th Symposium on Lactic Acid Bacteria, Egmond Aan Zee/Olanda, 31august-04 septembrie
  4. Sarbu I.,  Tanase A.M., Zamfir M.,  Vassu T., Stoica I., Chifiriuc C.M., Ionescu R., Pelinescu D., 2014, Screening for antimicrobial activity of lactic acid bacteria strains isolated from different Romanian food products, 3rd International Conference on Clinical Microbiology and Microbial Genomics, Valencia/Spania, 24-26 septembrie
  5. Sarbu I., Ionescu R. Vassu T., Stoica I., Ditu L.M., Marutescu L., Chifiriuc C.M., Petrut S., Pelinescu D., 2014, Study of some lactic acid bacteria strains probiotic effect  for use in gastrointestinal infections, 3rd International Conference on Clinical Microbiology and Microbial Genomics, Valencia/Spania, 24-26 septembrie
  6. Chifiriuc M.C., Marutescu L., Ionescu B., Ionescu D., Gheorghe I., Czobor I., Mihaescu G., Lazar V., 2014, Flow cytometry characterisation of gram positive and gram negative bacterial strains exhibiting non-enzymatic and enzymatic resistance mechanisms, 14TH EUROCONFERENCE ON CLINICAL CELL ANALYSIS, Lisabona, Portugalia, 13 -18 septembrie 2014.
  7. Zamfir M., Cornea P.C., De Vuyst L., Grosu-Tudor S.-S., 2014, Biodiversity and biotechnological potential of lactic acid bacteria, International Conference "Agriculture for Life, Life for Agriculture, Bucuresti/Romania, 5-7 iunie – prezentare orala
  8. Zamfir M., Grosu-Tudor S.S., Stancu M.M., Cornea C.P., De Vuyst L., 2015, Biodiversity of lactic acid bacteria from traditional Romanian fermented foods. Conferință invitată la European Biotechnology Congress, București, 7-9 mai
  9. Zamfir  M., Stancu  M.M., Stefan  I.R., Grosu-Tudor S.S., 2015, Bacteriocins produced by lactic acid bacteria isolated from Romanian traditional fermented foods. BioMicroWorld 2015, Barcelona, 28-30 octombrie 2015- Prezentare orală
  10. Biochemical properties and viability of two thermophilic lactic acid bacteria in a single chamber gastrointestinal tract simulator- GSI1: partial characterizations, 2015, Lucrări Științifice- Seria Zootehnie- University of Agricultural Sciences and Veterinary Medicine, Iași
Premii:
Partener USAMVB - autor Emanuel Vamanu
  1. Medalie de aur - Salonul Internaţional de Invenţii de la Geneva (ELVEŢIA 2013)  GIS1 – Sistem unicameral de simulare in vitro a tractului gastrointestinal uman
  2.  Medalie de aur –Expoziția Europeană a Creativității și Inovării (Iasi 2015). GIS2 – sistem in vitro de simulare a colonului uman în trei segmente










Results phase IV/2015

In last decades studies regarding probiotic products for improving the health of the consumer, were focused on obtaining new probiotic strains and highlighted the complexity of interactions between probiotic microorganisms and the human host (including microbiota of the gastrointestinal tract), finding that  positive effects were usually strain specific. The major goal of this project was to obtain strains and consortia of lactic acid bacteria with enhanced probiotic effect.
The operational objectives of phase IV-2015 were focused on: characterization of antimicrobial compounds produced by lactic acid bacteria and influence on culture conditions; the influence of consortia on human microbiota simulated in GIS2 system; chemically analysis of samples taken from GIS2 system; quantitative analysis of the compounds released by the epithelial cells in presence of lactic acid bacteria consortia, the cytotoxicity assay and the taxonomic identification of lactic acid bacteria strains by molecular techniques.
All objectives proposed in this phase have been achieved and 3 consortia were selected out of the 10 consortia obtained in the previous stage. The 3 selected consortia presented antimicrobial activity and enhanced immunomodulatory effect.
Some of the results were disseminated through publication in 4 ISI papers (3 of them were accepted and one was published), 2 BDI papers, 1 patent and 4 participation in international conferences. It is it worth mentioning that the GIS2 system was awarded with the gold medal at the salon EUROINVENT 2015.

Scientific publications:
  ISI journals
  1.  Grosu-Tudor S.S., Zamfir M., Van der Meulen R., De Vuyst L., 2013, Isolation of novel homopolysaccharide-producing lactic acid bacteria from Romanian raw milk and fermented dairy products, European Food Research and Technology 237:609-615.
  2. Vamanu E., Pelinescu D.R., Avram I., Nita S., 2013, An in-vitro evaluation of antioxidant and colonic microbial profile levels following mushroom consumption, BioMed Research International http://dx.doi.org/10.1155/2013/289821
  3. Vamanu E., 2014, Testing in vitro viability of thermophilic probiotic strain in simulated gastrointestinal conditions,  Annals of Microbiology 64(3):1439-1442
  4. Grosu-Tudor S.-S., Stancu M.-M., Pelinescu D.,  Zamfir M., 2014, Characterization of some bacteriocins produced by lactic acid bacteria isolated from fermented foods, World Journal of Microbiology and Biotechnology 30: 2459–2469.
  5. Vamanu E., Sarbu I., Nedelcu I., Pelinescu D., 2015, Study of PROEXO product influence on infant microbiota in an in vitro colonic fermentation system, Annals of Microbiology 65(2): 1189-1193.
  6. Ditu L. M., Chifiriuc M. C., Pelinescu D., Sarbu I., Pircalabioru G., Mihaescu G., 2014, Class I and II Bacteriocins: Structure, Biosynthesis and Drug Delivery Systems for the Improvement of their Antimicrobial Activity, Current Proteomics  11: 121-127.
  7. Gatea F., Teodor E., Paun G., Matei A., Radu G. L., 2015, Capillary Electrophoresis Method Validation for Organic Acids Assessment in Probiotics, Food Analytical Method 8 (5): 1335-1340.
  8. Moroeanu V. I., Vamanu E., Paun G., Neagu E., Ungureanu O. R., Eremia S. A., Radu G. L, Ionescu R.,  Pelinescu D. R, 2015,  Probiotic strains influence on infant microbiota in the in vitro colonic fermentation model GIS1, Indian Journal of Microbiology, 55(4): 423-429
  9. Vamanu E, 2015,  Effect of gastric and small intestinal digestion on lactic acid bacteria activity in a GIS1 simulator, Saudi Journal of Biological Sciences– Elsevier, on-line paper
  10. Sarbu I., Vassu T., Stoica I., Marutescu L., Chifiriuc C.,  Pelinescu D., 2015,  Phenotypic and genotypic assessment of Lactobacillus plantarum influence on Candida albicans fluconazole resistance,  Annals of Microbiology, 1-7.
  11. Zamfir M., Stefan I., Grosu-Tudor S. S., 2015,  Influence of growth medium composition on the bacteriocin activity of some lactic acid bacteria, Romanian Biotechnological Letters, in press
 IDB journals
  1. Ditu L.M., Chifiriuc M. C.,  Marutescu L.,  Bleotu C.,  Pelinescu D.,  Bezirtzoglou E., Mihaescu G., Lazar V., 2014, Immunomodulatory effect of non-viable components of probiotic culture stimulated with heat-inactivated Escherichia coli and Bacillus cereus on holoxenic mice, Microbial Ecology In Health & Disease 25, p.1-6.
  2. Ditu L. M., Chifiriuc M.C., Pelinescu D., Sarbu I., Lazar V., Mihaescu G., 2014, Two-Component Regulatory Systems – implication in the quorum sensing mechanisms and bacteriocin production in lactic acid bacteria, Biointerface Research in Applied Chemistry  4(4), p. 813-815.
  3. Zamfir M., Cornea P.C., De Vuyst L., Grosu-Tudor S.-S., 2014, Biodiversity and biotechnological potential of lactic acid bacteria, AgroLife Scientific Journal 3(1), p. 169-176.
  4. Vamanu E., 2015, Biochemical properties and viability of two thermophilic lactic acid bacteria in a single chamber gastrointestinal tract simulator –GIS1: partial characterizations, University of Agricultural Sciences and Veterinary Medicine Iasi, Lucrări Ştiinţifice-Seria Zootehnie, 312 – 315.
  5. Sarbu I., Vassu T., Stoica I., Chifiriuc C. Bucur M., Rusu E., Pelinescu D., 2015, Analysis on the antimicrobial activity of some lactic acid bacteria strains, Journal of Infectious Diseases 18(2-3): 87-91.
  Congress presentations
  1. Grosu – Tudor S.S., Zamfir M., 2012, “Functional properties of lactic acid bacteria isolated from Romanian fermented vegetables”, II International Conference on Antimicrobial Research, Lisabona, Portugalia, 21-23 noiembrie 2012, p. 563.
  2. Grosu-Tudor S.-S., Stancu M.-M., Pelinescu D.,  Zamfir M., 2014, Characterization of some bacteriocins produced by lactic acid bacteria isolated from fermented foods, 11th Symposium on Lactic Acid Bacteria, Egmond Aan Zee/Olanda, 31august-04 septembrie
  3. Sarbu I., Vassu T., Stoica  I., Marutescu L., Chifiriuc C., Nedelcu I., Pelinescu D., 2014, The influence of lactic acid bacteria strains on the antifungal resistance mechanisms of Candida albicans strains, 11th Symposium on Lactic Acid Bacteria, Egmond Aan Zee/Olanda, 31august-04 septembrie
  4. Sarbu I.,  Tanase A.M., Zamfir M.,  Vassu T., Stoica I., Chifiriuc C.M., Ionescu R., Pelinescu D., 2014, Screening for antimicrobial activity of lactic acid bacteria strains isolated from different Romanian food products, 3rd International Conference on Clinical Microbiology and Microbial Genomics, Valencia/Spania, 24-26 septembrie
  5. Sarbu I., Ionescu R. Vassu T., Stoica I., Ditu L.M., Marutescu L., Chifiriuc C.M., Petrut S., Pelinescu D., 2014, Study of some lactic acid bacteria strains probiotic effect  for use in gastrointestinal infections, 3rd International Conference on Clinical Microbiology and Microbial Genomics, Valencia/Spania, 24-26 septembrie
  6. Chifiriuc M.C., Marutescu L., Ionescu B., Ionescu D., Gheorghe I., Czobor I., Mihaescu G., Lazar V., 2014, Flow cytometry characterisation of gram positive and gram negative bacterial strains exhibiting non-enzymatic and enzymatic resistance mechanisms, 14TH EUROCONFERENCE ON CLINICAL CELL ANALYSIS, Lisabona, Portugalia, 13 -18 septembrie 2014.
  7. Zamfir M., Cornea P.C., De Vuyst L., Grosu-Tudor S.-S., 2014, Biodiversity and biotechnological potential of lactic acid bacteria, International Conference "Agriculture for Life, Life for Agriculture, Bucuresti/Romania, 5-7 iunie – prezentare orala
  8. Zamfir M., Grosu-Tudor S.S., Stancu M.M., Cornea C.P., De Vuyst L., 2015, Biodiversity of lactic acid bacteria from traditional Romanian fermented foods. Conferință invitată la European Biotechnology Congress, București, 7-9 mai
  9. Zamfir  M., Stancu  M.M., Stefan  I.R., Grosu-Tudor S.S., 2015, Bacteriocins produced by lactic acid bacteria isolated from Romanian traditional fermented foods. BioMicroWorld 2015, Barcelona, 28-30 octombrie 2015- Prezentare orală
  10. Biochemical properties and viability of two thermophilic lactic acid bacteria in a single chamber gastrointestinal tract simulator- GSI1: partial characterizations, 2015, Lucrări Științifice- Seria Zootehnie- University of Agricultural Sciences and Veterinary Medicine, Iași

 AWARDs:
Partner USAMVB - author Emanuel Vamanu
  1. Gold medal - Exhibition of Inventions  Geneva (Switzerland 2013)  GIS1 system – Single chamber system for in vitro simulation of the human gastrointestinal tract
  2. Gold medal -  European Exhibition of Creativity and Inobationn (Iasi 2015) – GIS2 – In vitro simulation system of human colon in three stages



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