Rezultate etapa V/2016
Principalul obiectiv al acestei etape a
constat în evaluarea funcţionalităţii consorţiului bazat pe tulpini de bacterii
lactice (BL). Pentru atingerea acestui obiectiv au fost parcurse o serie de
etape care au vizat: analiza influenţei
consorţiului asupra microbiotei umane simulate în sistemele GIS2; analiza din
punct de vedere chimic a probelor prelevate din sistemele GIS2; analiza
calitativă şi cantitativă a influenţei consorţiului de BL asupra capacităţii de aderenţă a microorganismelor
patogene la tumori prelevate de la nivelul tractului gastrointestinal;
identificarea taxonomică a BL din consorţiu prin tehnici de biologie
moleculară; evaluarea parametrilor biologici şi stabilităţii caracteristicilor
probiotice în diferite condiţii de conservare şi stocare; îmbunătăţirea
modelului experimental pentru obţinerea de produse probiotice complexe şi
pentru anticiparea activităţii probiotice in vivo.
Obiectivele propuse în cadrul acestei
etape au fost atinse şi au permis finalizarea proiectului cu selecţia unui
consorţiu de BL cu efect probiotic. Consorţiul de BL, alcătuit din tulpinile: Lactobacillus (L.)
casei, L. fermentum şi L. plantarum, a fost retestat privind
capacitatea probiotică în vederea stabilirii condiţiilor optime de conservare.
Rezultate au fost diseminate prin
publicare în 4 articole ISI (2 articole publicate şi 2 acceptate), un articol
BDI, 2 participări la manifestãri
ştiinţifice internaţionale si 2 brevete
nationale.
Results
phase V/2016
The main objective of this phase was to assess the
functionality of probiotic lactic acid bacteria consortium.
To attain this major aim of the project, the research
activities performed were focused on: influence of consortium on human
microbiota simulated in GIS2 system, chemical analysis of samples taken from
GIS2 system, qualitative and quantitative analysis of consortium’s influence on
adherence ability of pathogenic microorganisms to human gastrointestinal tract
tumors, taxonomic identification of consortium’s lactic acid bacteria strains
by molecular techniques, evaluation of biological parameters and stability of
probiotic characteristics in various conditions of preservation and storage,
improving the experimental model in order to obtain complex probiotic products
and to prepare in vivo tests for probiotic effect.
In conclusion, all the objectives of this stage were fully
accomplished and one consortium was selected out of the 3 consortia obtained in
the previous stages. The probiotic lactic acid bacteria consortium consisting
of Lactobacillus (L.) casei, L. fermentum şi L.
plantarum was retested for probiotic effect and to establish the optimal
conditions of storage and preservation.
The results were disseminated through publication in 4 ISI
papers (2 of them were published and 2 were accepted), 1 BDI paper, 2
participation in international conferences and 2 national patents.
Publication list
A.
Scientific articles published in ISI journals
1.
Mureşan A., Sârbu I.,
Pelinescu D., Ionescu R., Csutak O., Stoica I., Rusu E., Vassu T., 2016, In vitro selection of some lactic acid
bacteria strains with probiotic potential. Romanian
Biotechnological Letters, 23 (4). http://www.rombio.eu/published.html.
2.
Mureşan A., Sârbu I.,
Pelinescu D., Ionescu R., Csutak O., Stoica I., Rusu E., Vassu T., 2016,
Virulence profiles of pathogenic bacterial strains isolated from different
sources. Biointerface Research in Applied
Chemistry; Vol. 6. http://biointerfaceresearch.com/?page_id=1657
3.
Zamfir M., Stefan I.R., Stancu M.M, Grosu-Tudor S.S., 2016. Production,
mode of action and sequencing of the corresponding gene of a bacteriocin
produced by Lactococcus lactis 19.3.
Int J Food Sci Technol, 51: 2164–2170.
4.
Rusu E., Sârbu I.,
Cristescu C., Coculescu B.-I., Petruţ
Ş., Mureşan A., Vassu T., Pelinescu D., 2016. Highlighting the
antimicrobial activity of organic compounds isolated from some strains of
lactic acid bacteria, Revista Romana de Chimie, nr. 12.
B. Journals included in flow international BDI
1.
Vamanu Emanuel, The effect of a consortium
probiotic administration on a microbiota microbiological stability, Lucrări
ştiinţifice Seria Agronomie, vol.
59/2016, pg. 1-4
C.
Scientific papers presented
at
international congresses / published in abstract
1. Zamfir M., Stancu M.M., Ștefan I.R., Grosu-Tudor
S.S. Characterization of a nisin-like bacteriocin produced by Lactococcus lactis 19.3, isolated from
raw milk. Food Microbiology,
Birmingham, UK, 7-10 august 2016, poster. – si de la proiectul
PN-II-RU-TE-2014-4-0137
2. Petruț S., Pelinescu
D., Dițu L.M., Ionescu R., Vassu T., Stoica I., Chiciudean I., Tănase A.-M.,
Sârbu I., 2016. Comparative
studies regarding probiotic effect between individual/consortia of lactic acid
bacteria strains. “Power of
Microbes in Industry and Environment 2016” Symposium, 28 Septembrie- 1
Octombrie, Krk, Croația.
D. Patents
1.
Vamanu E., 2016, In vitro method for
testing the viability of lactic acid bacteria probiotic strains during
gastrointestinal tract transit, and unicameral system for its implementation.
2.
Vamanu E., 2016 A method of in vitro
testing of the probiotic strains viability during colon transit and system for
carrying out the method.
